... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...children. When I had my baby, we became closer. She supported me and answered my questions and honestly, she...

...histones and chromatin architecture to every diagram portraying some aspect of eukaryotic DNA would become busy...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

...producing long unspecific RNA fragments that are incorporated into the dsRNA necessary for RNAi. The Vellai...adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides into the dsRNA leading...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific...

...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...However, the theoretical brightness ignores several important experimental conditions that are related to the...

...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ... enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long...

...as well. In a screen, you aren’t killing off a portion of the cells like in a selection. Instead, all ... genome, and scientists have found that when a portion of the gene is deleted, it creates a non-functional...Complementing this mutation by expressing this deleted portion, for example on a plasmid, creates a functional...

.... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

...is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of ...efficiency of inducing double strand breaks and more opportunity for homologous recombination. Before CRISPR, ...versions of this out there. Addgene: Why was it important to you to make these plasmids available to other...

...nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop...knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown that the SAM system is robust...size of the backbone, transformations require electroporation. References & Further Reading: For more ...

...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...potential conflicts that waste money and time. Most importantly, “the company does not become real until someone...Synthetic Biology? Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup...

...transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation... chimeras For each protein-of-interest, it is important to determine which terminus can accommodate the...

...with their planned raises, some are taking the opportunity of the lapsed mandate (or are being compelled... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...

...rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for... Eisenhoffer lab, is a new tool that increases temporal and spatial precision of gene expression and reduces...

...almost any career. It allows you to share and build support for your ideas, invite others into decision making...Averaging ten panels each. (For this story, it is important to know that the student presenting right before...your work, opens up collaboration and growth opportunities, and gives your entire audience a chance to ...

...know about the vendor and the vendor’s technical support? An antibody from a vendor that you use frequently... with a responsive and knowledgeable technical support team, is a safer bet than one from a large distributor... know that application validation is the most important factor in antibody selection, but what does that...

...conclusions. Being vigilant about these errors is an important part of maintaining the quality of reagents, especially... especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are ...plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing. This platform sequences the entire...

...that the body’s architecture and plumbing are important. We started with learning the head is connected...and the G protein (supplied in trans) will be incorporated into the viral envelope (but not the viral genome...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

...Professor at Boston College. National reports stress the importance of providing authentic research experiences... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

...New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab, high school...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...vectors are available? What you’re building is important — but so is where you’re building it! Table 1 ...