We narrowed to 644 results for: rest
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TypeBlog PostPublishedAug. 10, 2023, 1 p.m....noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include:...scientists to focus on specific cell populations of interest. Many of the calcium sensors described are also...
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Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....SpCas9. gRNA A gRNA targeting a DNA sequence of interest is expressed in 293T cells and loaded into Nanoblades...transduction for most cell types they tested. Interested in using Nanoblades? Plasmids are available from... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....particles are delivered into the brain region of interest. AAV or lentivirus? Both AAV and lentiviral vectors...however, the spreading of vector to the area of interest may be very limited with this method, especially... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....outskirts of Guangzhouon west of beautiful Mao Fengshan Forest Park. As a national science and technology park...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27). Joined... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m.... are all wired up and expressing your opsin of interest, it’s time to get to the fun stuff: the experiments...Jeanne Kreek at the Rockefeller University. His interests include the role of learning and memory in opioid... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors to VP1,...into a compartment encapsulating the protein of interest (Figure 3). Figure 3: Schematic representation... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....student at Harvard University. Do you have a gene of interest but have run into a wall trying to study it? It...the Classical Genetics Simulator website. One interesting thing to know is that males do not recombine ... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....selected to preferentially target your cell type of interest. Viral pseudotyping is also a powerful tool that... optimize them for targeting your cell type of interest. For in vivo imaging applications, the ability... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m....DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....localization of the FP (fused to the protein of interest), thus tracking the localization of that biomolecule...at Albert Einstein College of Medicine. He is interested in everything related to gene expression, particularly... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...active in various gram-negative bacterial hosts of interest, including within mixed communities and into species... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “...would be complete without the inclusion of some interesting case studies, and Zimmer provides several. In... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....scientists can make mutations throughout a gene of interest based on an organisms codon usage bias to increase...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful...