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Showing: 401 - 420 of 511 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ... only lead to a better understanding of disease onset, but also better simulate a disease in a model system... custom TF programs to acutely initiate disease onset, providing both a tool to test prophylactics on ...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...re building software will help you be a valuable asset to the scientists as well as the developers. Lastly...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...

  6. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

  7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations in...

  10. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use...Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher titer...

  11. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ... website so scientists can view public circRNA datasets and download the custom python scripts needed ...phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to replace the drug...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...while lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly...

  18. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...different names depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical...practice, you may be able to do your compensation setup on your experimental samples without needing to ...

  19. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...). Misek and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic...by bias within the design of the experiment or dataset itself. It is important for scientists to not only...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across 8,792 organisms...biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...
Showing: 401 - 420 of 511 results