We narrowed to 489 results for: vit;

1. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...Trapnell. "Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy." Journal...

2. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through physical...

3. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...underlying causes of this unreliability: cross-reactivity (binds to non-target proteins), variability (...

4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression...

5. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...how to keep the replication of virus and its infectivity in check. Engineering controls, safety systems...

6. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...functions. Most individual parts have characterized activities with predictable behaviors. While a gene is the...

7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ... disease. To do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

9. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow...

10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience...

11. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

12. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...

14. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...

15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

18. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...