We narrowed to 868 results for: REN

...image provided by Manuel Gonçalves; made with BioRender.   Delivering gRNA and Cas9 using adenoviral...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow...

... the heat. Isolating male and female flies Differentiating male and female flies is essential for setting...their adult phase. It is important to clear out the parent crosses from the container before the progeny begin...thorax to look like stubble (sb). For a better reference of various fly phenotypes, check out the Classical...

...most cases, by using monomeric FPs or FPs from different families, which cannot form heterodimers (i.e....at the National Institutes of Health and he is currently an Associate Professor at Albert Einstein College...avid long distance runner, gardner, and cook.   References 1. Snapp, E.L., et al., Formation of stacked ...

...outcomes can be most effectively achieved with different CRISPR components. Select the appropriate CRISPR...cell lines is quite broad and includes several different methods, so we've summarized some key features...vitro cell culture. The user should review the current literature about their preferred model.  If your...

...expression of Cas9/gRNA and can be used for most of the current genome engineering applications of CRISPR including...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...Purification Good for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein...protein as pure as possible? Vectors that offer different combinations of tags are readily available, and...information and some protocols can be found in the references provided. Additional resources: Young CL, Britton...

... the Howard Hughes Medical Institute. Are you currently on or planning to go on the academic job market...if your model is correct or your data favor a different model. Finding evidence for or against a model...the answer to your research problem. Creating a coherent unified story and model can help make your ideas...

...jarring for the unprepared, and unfortunately, there aren’t a lot of resources out there for scientists making...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy immediately...

...writer? That’s okay! Your cover letter needs to be coherent and free of typos and grammatical errors. It does...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...experience, having given more than 20 talks at conferences and taught for five years at [name of the university...

... has been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week.... address the need to accelerate science using different approaches. We were also encouraged to see that...pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design, we were free to use multiple...

...remainder of this blog post. Perhaps the biggest difference between Cas13a and Cas9 is that Cas13a binds ...SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus, genotype human DNA, identify...Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O., et al. "C2c2 is a single-component...

...no papers, it's unlikely you'll get a job. The currency of scientific success is publications and grants...to convince me that their search committee is different and needs to be told all of the nuances of the...technologies, i.e. video interviews. For point of reference, I submitted my applications all by snail mail...

... blog post on Easi-CRISPR for more details. References and resources Want to know more? Check out the...Genome Editing General Homologous Recombination References: Repair of Strand Breaks by Homologous Recombination...Cas-mediated Transgenesis (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system...

...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...engineering in bacteria using homologous recombination." Current protocols in molecular biology 106.1 (2014): 1-...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...

...to a mouse model of muscular dystrophy. Three different splice site donor and acceptor sequences were ...for a transgene to be reconstituted, there is currently no one ideal split vector system. Overall the ...greater and more consistent transgene expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos...

...vegetable! What is an RNA aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

...Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...

...therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib... Does Not Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org...Models Using CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org...

...Fast forward a few hundred years and there are currently about 5.5 million articles in the English version...a team is a really good way of creating and strengthening those social bonds. So never mind how much better... you find there’s a need for an expertise your current team members don’t have - sometimes you just don...

... in the comments section below! Currently, there are over 20 different varieties of PA-FPs available to...function with extraordinary molecular detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz...

...click the "Save" button and jump around to the different pages by clicking on the appropriate boxes at ...at the bottom of the page before moving onto a different page so that any data you enter is not lost.   ...to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer" ...

... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...

...JEI. Chapter 3 - JEI target audience and their current readership. Encouraging students to do science ...biology protocols Check out our molecular biology reference pages ...

... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks...

...Top7 receives the Nobel Prize. Created with BioRender.com. Top7 gets its name from “topology of protein...David Baker, Demis Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani...

...research. Listen to learn more about Dr. Kwapata’s current work and thoughts on the need for more scientific...biotech research in Malawi 11:41-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44...

...the calculator in the comments section below. References 1. Choi, Vivian W., et al. "Production of Recombinant...Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed...

...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways... The AAV Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV...

...activated T cells, precluding analyses of T cell differentiation beginning with naïve T cells. The Framework...improving CD8+ T cell responses to cancer.   References and resources Milling, L. E., Markson, S. C.,...

... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening in...screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing a ...