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We narrowed to 807 results for: App;

Showing: 421 - 440 of 807 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... work on our external website appearance and functionality are happening and this strengthens our image...support more new projects. Diversification is happening. The Addgene team will have some fun challenges...

  2. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but it’s toxic to tissues...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-...

  3. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...that are not possible with traditional genetic approaches. In addition, the dTAG system is versatile and...update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in the meantime, please...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

  5. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...rates for AAVs are generally high enough for this approach to work, although it’s less effective in vivo ... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  6. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of...method is free for you, and many labs are quite happy to share, labs that get high volumes of plasmid ...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  7. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced...times) into the genome. Make sure to choose an appropriate promoter to express your cDNA, depending on the...flavors of activators which can be used with this approach - read more about them on our CRISPRa blog! ...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... after less than a minute of exposure to the appropriate light. The photoactivatable counterpart to this...protocols to get your CRISPR experiment moving. Happy editing! References and Resources References Hendel...

  9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...fluorescence that allow easy detection with the appropriate fluorescent illumination in in vivo, ex vivo,...of the most common tags currently used, are approximately 240 amino acids in size. The size of these proteins...Which tag should I use? When deciding on the appropriate tag, the experimental design should be the main...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...and Western Europe have taken three strategic approaches to gender equality over the past several decades...Australia worked with stem cells in mice. They appropriately used male and female mice (using both sexes ... the sex of the most commonly used cell lines appearing in American Journal of Physiology-Cell Physiology...

  11. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ... Tang and Cepko were eager to see if they could apply GFP regulation to other types of proteins. They ...systems are easily adaptable to neuroscience applications, including optogenetics, and they should make...Tang note that their scaffold system is likely applicable to many types of proteins. For proteins with ...

  12. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...tail clip, or toe clip) and using PCR with the appropriate primers to detect your targeting vector or gene...your parental mouse should be hemizygous at the appropriate allele. When crossed to a mouse that is homozygous...F1 heterozygotes to each other will generate approximately 1 in 16 mice that harbor homozygous alleles ...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...Scientists are currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal... 2019. For more information about the aims and approach of CeNGEN, check out their publication.   3’UTRome...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...

  14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...more about it on the blog. COVID-19 research is happening fast and we’re glad to join these efforts and ... JM, DiVenere AM, Kuo H-C, Javanmardi K, Le KC, Wrapp D, Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK,...resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction...

  15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO) focused on understanding...not related to this rescue function, GdnHCl was approved by the FDA in 1939 for the treatment of botulism...Given its unique molecular biology and previous approval by the FDA, GdnHCl could, in principle, provide...

  17. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...biomolecules. They can be part of multiple dynamic and overlapping complexes that have distinct functions. Many .... (2017). Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local protein interactomes by proximity‐dependent...

  18. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...of competitive TED talk – to win the audience’s applause with your performance. When your institute opens...guided tours to show people where the lab magic is happening. 3) Volunteer – Step into science education Do... Blog - Hone your writing skills Take the DIY approach and start a blog. Stellar talents like Ed Yong...

  19. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...protein in your sample? Or what if something weird happened during your western and your results look… funky...to probe the blot again, almost like new. This approach preserves your sample and saves you from having...retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...multigene constructs, through multi-TU assembly,happens via three steps: Level 0: Cloning of basic parts...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have...
Showing: 421 - 440 of 807 results