We narrowed to 577 results for: BASE

1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...application for OptoNBs takes us into cells: light-based control of signaling pathways. This is particularly...

2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

   Type

   Blog Post

   Published

   June 23, 2022, 1:15 p.m.

   ...0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics...

3. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...Found in surgical suites, air fields, and military bases, checklists are the ultimate tool for doing things...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   Published

   Jan. 21, 2025, 2:15 p.m.

   ...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...

5. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...expression.     AAV retrograde Unlike traditional dye-based neuronal tracing reagents, AAV particles require...

6. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

   Published

   March 30, 2023, 1:15 p.m.

   ...consistent with the cell type that was predicted based on the Atlas. They also looked at 17 of these TFs...

7. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...activation of transcription in bacteria by co-opting σ54-based transcription. Transcription from σ54 promoters ...

8. Components of CRISPR/Cas9

   Type

   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ... sequence contained within the guide RNA and a 3-base pair sequence known as the Protospacer Adjacent ...

9. Finding and Joining Your Dream Lab

   Type

   Blog Post

   Published

   April 8, 2014, 2:50 p.m.

   ...interests.” George Church selects new hires initially based on those who notice and embrace the “odd and extreme...

10. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    Published

    Dec. 20, 2017, 2:33 p.m.

    ...liability in respect to actions taken or not taken based on any or all the contents of this post.  An attorney...September 2017, USCIS received 116,224 employment based green card applications and approved 91,023 during...

11. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ... Knockout cell line approach Genetic knockout-based validation can conclusively prove the ability of... through its ability to separate the cell lines based on expression of the target. In this case, you could...

12. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...NIR photosensitizer that allows for precise light-based control of protein inactivation/cell ablation while...

13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results reported elsewhere...

14. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-...

15. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...specificity. This can be especially important for base editing techniques, where Cas9 must be directed ...

16. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune cells...

17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...wanted, the team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed...

18. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky...

19. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...that the antibody is guaranteed to work. Antibody-based assays are complex and antibodies may perform differently...

20. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...is one of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101...