We narrowed to 534 results for: CAR
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TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus on antibodies...
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Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....obvious component of a biosensor is the thing that you care about within your cellular or animal system. This... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....DREADDs were engineered from members of the human muscarinic receptor family and are activated by small molecule... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m....a transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....keep track of what works, and what does not work. Careful documentation will keep you on task and will prevent... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....result in a high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....laboratory animals during in vivo research and the CARE guidelines for case reports are two sets of guidelines... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m.... die unless they have incorporated the plasmid carrying the required gene. The table below lists some... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....different plasmid backbones that varied in size or carried different genes. In these cases, gene expression... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley Listen to the anti-CRISPR podcast segment... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....Epitope availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....proteins will refold once denatured, however, so take care when using this method. Fig 2: A, ... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m.... be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for integration at a...