We narrowed to 623 results for: Dos;
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TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...
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Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog PostPublishedFeb. 20, 2014, 4:57 p.m.... lentiviral CRISPR library. “This enables you to do customized genetic modification on a scale that was... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m..... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it? Test the serotype and promoter The first... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....of different cancers (Jin, et al. 2023). So where do all these sdAbs come from? In the early years of ... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m.... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....where affinity tags come in – they are peptides which do not naturally exist in most expression systems that... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....enveloped viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is ... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....learning, decision making, and motor control. What do we understand about Dopamine? DA is produced by specific... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m.... rhythmic movements like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....antibodies are excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....., Sun, H. et al. Hortic Res. (2020). I can do this by expressing a gene that rescues the mutations... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in biochemistry... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m.... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs)...