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...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

... lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was...

...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

.... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...

...of different cancers (Jin, et al. 2023).  So where do all these sdAbs come from? In the early years of ...

...high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in biochemistry...

... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of...

...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that...

...both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools...

...enveloped viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is ...

...antibodies are excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material...

...., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the mutations...

...EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable...

...learning, decision making, and motor control.  What do we understand about Dopamine? DA is produced by specific...

... rhythmic movements like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different...