Skip to main content
Addgene
Showing: 421 - 440 of 620 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    .... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

  2. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

  5. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...learning, decision making, and motor control.  What do we understand about Dopamine? DA is produced by specific...

  9. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...of different cancers (Jin, et al. 2023).  So where do all these sdAbs come from? In the early years of ...

  10. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in biochemistry...

  11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of...

  13. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the mutations...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that...
Showing: 421 - 440 of 620 results