We narrowed to 530 results for: Erf

1. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...researchers with useful and adaptable tools to perform basic and preclinical research. For scientists ...

2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9...

3. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ... study the mechanisms of infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab....

5. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment...

6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder...

7. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ...the resources to recapitulate the experiments performed by our thousands of depositors. That means their...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ... FoR 2015 Symposium Learn to Advocate with the Perfect Science Talk Find a Good Mentor ...

9. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...regions of the genome, we have deposited the best performing sensors and constructs encoding corresponding...

10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...findings from structural studies on the ribosome performed in his lab. RNA is a structural and functional...

11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... be open to a wider group of people. This is a perfect starter project for our computer scientists, many...

12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...problem by stating the facts and explain how it is interfering with your work. Avoid talking about your emotions...

13. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...good panel discussion is a well choreographed performance with room for fortuitous improvisation. You want...

14. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...of the skin. While this could theoretically be performed anywhere on the body, somewhere large, flat, and...

15. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help remove toxic...

16. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...hundreds of nanograms of DNA, you will likely need to perform several individual transformations, such as splitting...

17. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...corrected flow plot in 2A, the populations are not perfectly straight, but the center of each population is...

18. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...

19. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...cells. Baculovirus expression systems are very powerful for high level recombinant protein expression....

20. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...PMCID: PMC4777285. Further Reading Microscopy U. Generfal Considerations when Using Fluorescent Proteins...