...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...

...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ...

...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...about ELN costs and limited budgets, “a large percentage of survey respondents indicated that cost was...effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger, Tea Pavlek...

...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality...

...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real...

...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...help each other by sharing their data. So take advantage of it and get your data - negative AND positive...

...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

...the bench waiting for you, prep for research work has to be done by the researchers themselves.  Be ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ...

...There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone...

...experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen...Growth of new livers in culture could resolve this shortage." What is the knowledge gap you plan to address...

...two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct...

...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially...

...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at room temperature...

...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...genome-wide screens.   Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen...

...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...sequence and other regions of the genome. The main advantage of NGS is that it allows you to simultaneously...

...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking...

...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether...bd-spectrum-viewer.   But what is the real-life advantage of a brighter fluorophore? Strong brightness makes...

...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups...