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We narrowed to 450 results for: HA tag

Showing: 421 - 440 of 450 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

  2. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over...

  4. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl substitutions...

  9. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...of current methodologies. This section sets the stage for why your funded proposal will address these ...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...who are interested in such careers should take advantage of their academic environment and attend seminars...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...available from Addgene as of 2023. A large percentage of viral vector preps contain biosensors, optogenetic...Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category of viral vector...

  13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...about ELN costs and limited budgets, “a large percentage of survey respondents indicated that cost was...effort and give the lab a serious competitive advantage.  Many thanks to our guest blogger, Tea Pavlek...

  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over in videos...: If you’ve ever watched any behind the scenes footage in the last several years, you can usually see ...

  15. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...fluorescent molecules, including fluorescent proteins, has led to the alternative acronym Fluorescence Resonance... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based...

  16. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ... Strategize, choose calls that suit your career stage / research area, and focus on producing a good quality...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...know this?” Over the past fifteen years, the CBM has developed a student-centered SMART Team modeling ... of proteins using 3D printing technology. The tagline for our company is “…where molecules become real...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...Naldini,L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6...

  19. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...help each other by sharing their data. So take advantage of it and get your data - negative AND positive...

  20. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone...
Showing: 421 - 440 of 450 results