We narrowed to 853 results for: IGH@

...bigly. But such a project should not be undertaken lightly. Whether executed in arrayed or pooled format there...are just as critical for success. Sometimes, you might have a few positive control genes on which to test...execute genome-wide perturbations. So, while some might argue that the only way to do something is to do...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...instance, you might use it as part of a flex switch you’d like to work with in neurons or you might use it in...Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course...brain. There are many possibilities enabled by this highly modular AAV - that’s probably why it’s so popular...

...group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out together. “We also ... celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes...

...? If so, you might want to find additional sources of wisdom. While somewhat lighthearted, the article...article does highlight key characteristics of a good mentor: Yoda image courtesy of jorenl/Vecteezy.com...

...share? And how they are connected? Here are the highlights written as 8 tweetable facts. Note: The images...most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on Twitter. ...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist? If you ...

...them share their reagents, we knew our role in fighting the pandemic could be even more impactful by reaching...scientists working in a for-profit setting.  In light of the need for basic research during the current...University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership with...

...Agrobacterium, along with a selectable marker. Left and right T-DNA borders specify the region of DNA that will...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in both...that gene expression from the M24 promoter is much higher than that from the commonly used 35S promoter. ...transfer. The M24 promoter provides constitutive, high-level gene expression and is functional in a variety...

...been in space is pretty small). Therefore, I was delighted to be invited to the ceremony for surely what ...non-scientist from Bloomberg news. Given the topic it might have been better to have a scientist up there, but... viruses to give information to the computer to fight the known viruses. Bacteria have figured out how... virus sequence to enable them to recognize and fight it forever. He emphasized the potential of this ...

...Gates’ Foundation is involved with was one of the highlights of the conference. It was inspiring to see how...trusty spray bottles! We also raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky...Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening...

...toxic to both lines, it probably has either high toxicity or high off target effects, both of which make it... druggable target). Diseases with defined, straightforward causes (a specific mutation in a known gene...targets by CRISPR can accelerate the drug pipeline. High-throughput CRISPR screens can be used to inhibit...

...research". The family entered the biomedical field’s spotlight after researchers discovered heavy-chain only ...reading frame Ability to access unique epitopes with high affinity, due to a greater ability to target concave...cellular functions, like in this early example we highlighted back in 2015, where sdAbs were used to create...

...skills to get the work done efficiently and with high quality. Their work involves coordination and can...t mean going rogue, pointing out problems or highlighting criticisms. To be given the opportunity to take... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...

...planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO Cloning...and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary...efficiency).  5. Select and Analyze 10 White or Light Blue Colonies: You can confirm the presence of your...

...isotype converted, or chimeric, antibodies may be the right solution for you. Read on to learn more. What is...is an isotype? Antibodies are composed of light chains and heavy chains made up of variable regions and...097037-0.00017-8     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J...Polyclonal Antibodies Antibodies 101: Selecting the Right Antibody  ...

...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available ...absolutely delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights!    Table...

...way? What ecosystem makes your heart sing with delight? From developmental biology to developing a career...example, how to use new technologies to reach the right audiences and how open access affects financial ...of scholarly publishing. And see for example the Eighth International Congress on Peer Review and Scientific...interested in the science communications world, you might consider an internship or a short program to build...

...neuroscience with ever more efficient tools for light mediated neuronal control and we are expanding our...target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids...apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent...glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg...

... Addgenie Julian Taylor-Parker explains why you might want to use a specialized E. coli strain for protein... to an entirely new career path, a career coach might be able to help you. Head Addgenie Joanne Kamens...genome, you need to check whether you’ve made the right one. In this post, Addgenie Melina Fan guides us...guides us through some common controls and why you might need them. Happy Reading!   Resources on the Addgene...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well ...Like a considerable number of researchers, I had high hopes of securing tenure in a British or Irish University...interviewed for 1 hour so having a broad enough insight was going to help me push both my scientific and...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

...amplification and verification processes we use to ensure high-quality library distribution. You can also use these...amplification 101 Library amplification seems straightforward: E. coli bacteria are transformed with the ...a typical library, Addgene usually does four to eight electroporation reactions using a total of ~400ng.... Analyzing the reads is, again, seemingly straightforward – you need to count how many times each of ...