Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 708 results for: MAK;

Showing: 421 - 440 of 708 results

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

  4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...transfer office or other authorized office directly to make sure the deposit agreement/MTA is taken care of ...spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries with...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

  9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

  11. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...chromoprotein detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if ...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

  16. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

  17. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to ...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...accurate, as these peptides are thought to function by making the ribosome skip the synthesis of a peptide bond...within the same multicistronic vector, effectively making a construct that expresses multiple unique genes...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...or validating your new clones, try these tips to make the most of your time and sequence! You might also...Standard Nucleotide BLAST page, the first decision to make is whether to compare a Sanger sequencing result...
Showing: 421 - 440 of 708 results