We narrowed to 666 results for: MAL

...contains only the evolved TadA and is ~500 base-pair smaller than ABE8.x-d.  This resulted altogether in 40...cell line for these versions they found that the smaller version, ABE8.x-m, showed comparable editing behavior...

...ligase fusion protein identifies proximal and interacting proteins in mammalian cells. The Journal of cell ...

...publish brief novel findings. These results are normally one figure and can be negative and/or even replicated...develop computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid...

... types of targets: primary cells, embryos, and animals. Nanoblades can also carry different types of CRISPR...delivery Learn about CRISPR delivery methods for mammalian cells Browse our CRISPR 101 blog post Resources...

... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...

...when the antibody is produced from a new set of animals), and wrong application (different experiments/...antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules), reliable (wouldn...

...persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV...

...'t do it unless you can do it well" – even the smallest program. I don't mean have fancy food or a fantastic... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

... never a “moment”. But there were a lot of very small steps. Life in a cutting-edge synthetic biology...analysis, and was a marketable product. It was a small project, not a full-blown career, but it was enough...

...five amino acids, meaning the tag could be quite small. However, substance P has an amide group added to...larger than the substance P peptide, but still quite small. However, unlike the substance P tag, they found...

...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

...dose (TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective...

...as the donor in HeLa cells.   Find ShadowR for mammalian cell expression here   Now that you’ve learned... J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., … Forster, A. C. (2018)....

... SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

... Figure 1 for commonly used markers for major mammalian organelles and transport pathways. It is best,...: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka, and John H. McDonald. "A practical...

...their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. ... timers should therefore find use in studies of animal development where events like the patterning of...

...improved protocol. Many scientists, publishers, and small start-ups are turning to software apps to solve ...you painstakingly complete your experiment – one small victory in a long road to your scientific breakthrough...

...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

...answer to the space problem. Instead of imaging smaller, what if we just made the sample bigger? In ExM...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

...to screen for small-molecule inhibitors of a particular PPI. In such experiments, small-molecule inhibitors...