We narrowed to 794 results for: Mpl

... resources and tools. That message was fully exemplified in Cori Bargmann’s keynote speech and in the ...vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016...Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared by the...Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression Map of an Entire Nervous System...

...titers measure the amount of viral particles in a sample and are usually based on the presence of a viral...however, is that a p24 ELISA measures all p24 in the sample regardless of whether or not it is incorporated...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...measures infectious viral particles. One of the si mplest methods of measuring functional titer uses FACS...

...modification is chromatin remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular...different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting. In imprinting...hypomethylation, on the other hand, has also been implicated in the development and progression of cancer ...have huge impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis...

...fluorescence when a small magnet was passed over the sample (Frank Hayward et al., 2024). The fluorescence ...intensity decreased whenever the magnet approached the sample (by less than 1%) and recovered after the magnet...expressing MagLOV while a magnet is waved under the sample. The imaged area measures approximately 82 mm across...Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian ...

... helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The ...purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of ...known titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

...signals are integral to many facets of biology. For example, many heart issues stem from the heart beating ...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...naturally occurring voltage indicator. To give an example, a genetically encoded phosphatase (voltage sensing...then localize and emit signal from. This specific example is attractive due to the large number of sheer ...

...difficulty answering questions. Or perhaps you’re simply attending, but uncertain how to capitalize on your...criticism, and ask thought-provoking questions. For example, if you’re a virologist presenting at the American...like to say to help build your presentation. For example, when thinking about discussing a figure, jot down...guilty of this, and have many times answered a completely different question than the intended one. Take...

...beam into a thin sheet to illuminate a fluorescent sample and captured the reflected light using a different...microns, illuminates only one thin plane of the sample surrounding the focal plane of the detection lens...multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it...problem is to illuminate and acquire images of a sample from two opposing sides and merge the images, such...

... of genes and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to... sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips... Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop.’, Proceedings...plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering Using arabidopsis in...

...tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed primers....increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and ... not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is ...

...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...does not require de novo viral gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, ...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...in vivo and maintain activated CD8+ T cells: implications for their use as vaccines. Blood, 110(6), 1916...

...Technique What It Locates ChIP-seq Proteins (for our example later, histones) ChiRP-seq RNA Hi-C DNA (genome-to-genome...appendix DNA is a 3D fractal). Enhancer biology is a complex and dynamic field. We’re going to focus on a tried...People are wrong. In reality, cellular DNA is a complex three-dimensional globule of chromatin. Chromatin...Bedtools getFasta. These regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then...

...Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using...structures of CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide and ...on its own, with drug ligands, and in ternary complexes with other proteins, establishing CRBNmidi as ...first use of CRISPR/Cas9 in M. abscessus. The simplicity and efficiency of this dual-plasmid approach ...

... and the complexity of effectively deploying them in the lab somewhat mirrors the complexity of understanding.... Addgene’s Viral Vector Service Besides being complex to use, viral vectors are also difficult both to...broadly useful vectors, a process that isn’t as simple as it seems. Take, for instance, the GCaMP-encoding...

...within the scientific community, as they allow for simplified transformation processes, and can be used by ... the others are functional in other hosts. For example, the pJD4 plasmid harbors distinct origins (ori1... load (Meyer 2009). Figure 1: Idealized examples of a narrow host range plasmid with only one ori... hosts in which proteins can be expressed. For example, scientists can incorporate interchangeable antibiotic...

...sequence of interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription...RT-RPA), respectively. Amplified nucleotides are combined with Cas 13a in complex with crRNA targeting ...

...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...that there are some genetic factors involved. For example, multiple studies show that certain mutations of...positive potential for treating mesothelioma. For example, patients who have peritoneal mesothelioma – that...immunotherapy that work in different ways. For example, a new drug known as pembrolizumab (Keytruda) causes...

...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA... the 20 amino acids and three stop codons. (B) Example of codon usage among different organisms. There...prokaryotic systems or yeast (Lanza et al., 2014). For example, it may be worth codon optimizing a human gene ...optimized for expression in mammalian cells. For example, Henry Lester’s lab optimized the C. elegans GluCl...

... list of specific structures within cells. For example, to improve nuclear localization of fluorescent...//doi.org/10.1101/160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits...Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion body problem...problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!  First, the...

...only occur through editing by a APOBEC-Cas9n-UGI complex. So how exactly does this work? Figure 1:...DNA editing efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create...DNA editing efficiency of APOBEC-Cas9 editisome complexes. The eGFP reporters rely on the correction of ... single base editing by APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038...