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We narrowed to 751 results for: Nes;

Showing: 421 - 440 of 751 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...system, in which the required adenoviral helper genes are supplied on two different packaging plasmids...pHelper plasmid containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing replication ...replication factors and serotype-specific capsid genes.  In this helper-free system, HEK293T cells, expressing Ad...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...The degradation tag (dTAG) system functions by harnessing the power of a novel degrader of the 12 kDa protein...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...all the tags discussed here and profile their effectiveness with over a dozen different proteins. In the...

  3. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

  4. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner, you...also quite efficient; although efficiency often declines as the number of fragments you’re hoping to assemble...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

  5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...Manuel Leonetti to tag all 22,000 protein coding genes in the human genome with fluorescent proteins. For...tool Crispor by Max Haeussler. With a few extra lines of code, we enabled it to return guides and primers...which is important for quality control. The only ones we found that worked with NGS data were: Crispresso...

  6. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available...measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791. https...

  7. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...are millimeters in thickness (8,9).  In addition, there is a drop in image brightness with increasing depth... Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view imaging...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...should be run in duplicate or triplicate. The closeness of the data points generated by replicate values...be linear or nonlinear. BCA assays have linear trendlines while those for ELISAs and other enzymatic assays...advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind of software...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...you can learn more about possible strengths and weaknesses, and view everything as a learning experience... weaknesses...

  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...editing from the Cas13b REPAIR system To test the robustness of REPAIRv1, the lab targeted 34 pathogenic G...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...students learned something. Based on the initial guidelines I received from the teachers, I formulated the...own wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of Chenery Middle School for their tremendous...

  13. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...studying transcriptional and translational dynamics of genes and proteins in living plants, to complementing ...process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol, as ...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...method of tagmentation for next-gen sequencing pipelines (e.g. using Tn5), and as knock-in tools for human...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry and Biochemistry...my students published their work identifying new genes required for stem and progenitor cell maintenance...for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014; Duboue et al., 2022...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...hybrid system (also called the interaction trap), genes encoding proteins of interest(POI’s) are fused to...variations, including many that use auxotrophic rescue genes as reporters and some that repress instead of activate... Vectors Read our Blog Post About Tagging Yeast Genes ...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...sharing information through the use of popular Chinese social media platforms.  As you can imagine, shipping...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...(Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous cleavage and degradation of neighboring...ability to cleave non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform...Kellner MJ, Tan AL, Paul LM, Parham LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel environments... (BDNF). BDNF is a small protein involved in neurogenesis, neuronal survival, and synaptic plasticity.... insights into the complex relationship between genes, neurons, and behavior. There are several other ...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...coat! These are the things the rest of the world imagines when they think “scientist.” But leaving the bench...that at a desk, it is much easier to stick to timelines. This is probably because you don't have to worry...transferable skills guide Read blog posts about career readiness Resources at Addgene.org Find careers at Addgene...
Showing: 421 - 440 of 751 results