We narrowed to 552 results for: PPL;

1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...

5. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

6. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

7. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...

8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...

9. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP...

10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

11. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

12. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...antibody and content team, we identified what applications should be included and what basic information...

13. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...require there to be a culture condition that can apply enough pressure to select for variants of interest...

14. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

15. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ...can also allow multiplexing with experiments that supply both substrates and measure luciferase output at...

16. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

17. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected...

18. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...

19. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...

20. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...