We narrowed to 599 results for: REV;
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TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....directly kill bacteria (bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction...
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Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....read more about what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....differences are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....short culture periods (less than 20 passages) to prevent these issues from popping up, and if your research... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m.... different about academia? Publishing in peer reviewed journals is absolutely necessary for success One... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make knockouts and other types... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....kick off at. Whatever you’re working on, start wherever it makes sense to start and try to be as chronological... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... and structural deformation, these probes are reversible and do not cause structural deformation or photo-induced... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology Target Discovery... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m....run that last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....ended up taking the ‘dead-end’ path. The CellTags revealed that cells with common ancestors tended to have... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....antibiotics. Simon Moore and Paul Freemont’s lab previously developed such a system in Streptomyces, a prokaryotic... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization assay... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (Bradbury & Plückthun, 2015a... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....mean? How did the researchers solve the problem preventing them from moving forward? Answering these questions... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m...., I had the chance to carry out a comprehensive review of the literature informing the notion of “heading... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m.... Stasevich lab tested several scFvs scaffolds previously proven to fold and function in living cells. ... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which results in...