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We narrowed to 837 results for: RING

Showing: 421 - 440 of 837 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ... allow cells to become persistently fluorescent during development. Stochastic multicolor labeling systems... tags instead of XFP to optimize it for tissue clearing, a method that makes tissues, such as the brain...visible. Chemical tags are more resistant to tissue clearing methods and can be labeled by synthetic fluorophores...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...tools this could be applied to. When it comes to altering gene expression, CRISPR technologies have gradually...-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA..._module_attribute %}{% end_module_block %}   Interfering in the future While RNAi has retreated a bit ... the background, it remains a solid option for altering the expression of specific mRNAs. shRNAs are a...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...biotin-streptavidin interaction (Figure 1a). This interaction brings the fluorophore in proximity to the target marker... used. These dyes are incorporated into the DNA during DNA synthesis and then diluted with subsequent ...negative live cells can still be easily gated on during the analysis. Conclusion Congratulations! You made...intracellular labeling protocol is necessary, requiring chemical treatment of the cells after surface ...

  4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... the cut site were not efficiently incorporated during HDR. We decided to systematically characterize ...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org...

  5. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...), and in SNP analysis (to introduce naturally occuring SNPs in a plasmid context). The technique is also...sequence critical for Cas9 cleavage), thereby rendering the resulting construct resistant to Cas9 induced... successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers should ...’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...

  6. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...assembly work? The required homology between neighboring fragments can be created via PCR amplification...including regions of homology to the vector or neighboring fragment. Then you would amplify the fragments...required single-stranded annealing and priming of neighboring assembly fragments. Gibson assembly is commonly...modular overlap-directed assembly with linkers) which brings modularity to overlap-directed methods (Casini ...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally, delivery...and/or its intermediate engineered precursor. Considering systemic capsids for your research? While systemic.../cJ and CBA/J mice. AAV1-X1 was developed by transferring the 7-mer peptide region from variable region...Fox AS. (2023). Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate...Bloom, J. M., Seed, C., & Deverman, B. E. (2019). Delivering genes across the blood-brain barrier: LY6A, a...AAV vectors to the central nervous system by engineering capsid–receptor interactions that enable crossing...

  8. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...recorded plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968...whole plasmid sequencing of our plasmid samples during our quality control process. Since MiSeq involves...D, Berg DE. pBR322-derived multicopy plasmids harboring large inserts are often dimers in Escherichia ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...An off-target rate of 5% is far too high when considering human patients and the diseases associated with...off-target frequency is (more on this below). Lowering off-target events There are several strategies...compatible with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want ...which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous...  Some classes of neurons transiently express TH during development or have low levels of TH mRNA without...time, frustration, and funds in the long-run by ensuring you are minimizing any off-target expression that...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in Vibrio cholerae that encodes...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  12. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...the five is different. To identify significant pairings, additional tests, referred to as post-hoc analysis...temperature, humidity, sunlight exposure time, and watering volume on total crop production (though you would... error is the probability of a false positive occurring when many comparisons are performed. For example...drawbacks to these tests as they can be quite stringent if many samples are assessed. However, if many...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad school, you searched...own experience, great mentors often can be found during conversations with senior investigators. Discussions...fundamental conflict. Science is about exploration, discovering new information. There are literally infinite...well practiced or too skilled at presenting and answering questions. Organizing a thoughtful engaging talk...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...letters and be able to guess whether or not that string of letters is a part of the word you’re searching...monoclonal antibodies, which come from naturally-occurring immunoglobulin genes, recombinant antibodies are...Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional scFv antibody in E. ... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ... best known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...PMCID:PMC528746. 5.Gurskaya, Nadya G., et al. "Engineering of a monomeric green-to-red photoactivatable ...organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...evidence,” and to speak out in unprecedented force during the 2015 federal election.   Even as we marched... of the few members of Congress who holds an engineering degree. It was a great, positive event that I...a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also ...face of evidence. Stephanie Hays is moving from engineering synthetic microbial communities at Harvard University...

  17. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author... holiday celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured...

  18. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...breakdown Intro Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging ...Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker...to introduce students to this amazing genome enegineering technology Resources at Addgene.org Find basic...

  19. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    Published
    Aug. 17, 2021, 1:15 p.m.
    ...a GIPHY page! The best part is, we’re not only sharing them with you, but you can share them with your...labmates, and friends as well!   Addgene is sharing so much more than plasmids these days. via GIPHY...out everything we’ve uploaded so far, and start sharing the science love today!        ...
Showing: 421 - 440 of 837 results