We narrowed to 490 results for: SUB
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TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....two-photon microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic...
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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....et al., 2024) have highlighted how chromatin substantially affects prime editing outcomes across many genomic... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 20 bp ... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish between... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....that you reach out to potential advisors before submitting your application. I found this step to be incredibly... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m.... do next to how much data is necessary before submitting a manuscript. In fact, how many of you have negotiated... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....vast majority of AdV-modified human cells being subjected to scarless homology-directed genome editing (... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....will be reviewed by an internal committee before submission to ensure that it does not divulge any propriety... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m..... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m.... anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition to the expected... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum where you... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....-coding sequence using a recoding strategy; a subsequent drive can restore the wild-type sequence (save... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....experiments. Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering as applied... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m.... global activity in our particular scientific subfields. Plasmid requests correlate with paper publication... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....use, it’s worth scheduling the second and other subsequent meetings. The number of participants, breadth... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....These pages provide helpful information about the subject, link to related guides and protocols, and provide... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...