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The Importance of a Fun Workplace: Company Culture at Addgene

Type

Blog Post

Published

Sept. 3, 2015, 2:30 p.m.

...something fun is going on and to get there fast! Spread word of your plans by email, gong, evite, posters...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

Published

Aug. 30, 2022, 1:15 p.m.

...Immunofluorescence Staining. Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

Published

Aug. 27, 2020, 1:15 p.m.

...Analysis of Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org...
A Guide to Starting Your Own Journal Club

Type

Blog Post

Published

Feb. 6, 2020, 3 p.m.

... a week for several months at a time during the spring and fall semesters. Our scheduling was in part ...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

Published

Sept. 8, 2016, 2:30 p.m.

...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

Published

March 17, 2016, 2:30 p.m.

...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

Published

May 15, 2014, 3:33 p.m.

... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

Published

April 11, 2017, 2:30 p.m.

... References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

Published

Aug. 14, 2018, 12:42 p.m.

...fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009): pdb-top63. PubMed...
Antibodies 101: Reading a Flow Plot

Type

Blog Post

Published

Feb. 6, 2024, 3:13 p.m.

... density of cells, while contour lines that are spread apart show a low density of cells (Figure 7). ...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

Published

July 25, 2019, 12:03 p.m.

...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

Published

July 23, 2019, 12:46 p.m.

...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Type

Blog Post

Published

Oct. 27, 2015, 3 p.m.

... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

Published

Oct. 1, 2015, 2:30 p.m.

...collaboration and the exchange of ideas. The rapid spread of optogenetics throughout the neuroscience field...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

Published

April 7, 2016, 2:30 p.m.

...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...
Plasmids 101: Control Plasmids

Type

Blog Post

Published

April 9, 2015, 3:29 p.m.

...or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

Published

April 3, 2014, 8:05 p.m.

... phosphoglycerate kinase gene. Constitutive Widespread expression, but may vary by cell type. Tends ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

Published

April 9, 2019, 1:13 p.m.

...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

Published

March 5, 2019, 1:08 p.m.

...Multicolor Brainbow imaging in zebrafish." Cold Spring Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson...
Plasmids 101: Biotinylation

Type

Blog Post

Published

Nov. 15, 2018, 1:50 p.m.

... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific...

Showing: 421 - 440 of 450 results

Results per page:

The Importance of a Fun Workplace: Company Culture at Addgene

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

A Guide to Starting Your Own Journal Club

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Fluorescent Proteins 101: Aptamer Fluorophores

Neuronal labeling with Spaghetti Monster

Antibodies 101: Reading a Flow Plot

Save Time with Transient Plant Leaf Transformations

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Important Considerations in Optogenetics Behavioral Experiments

Getting the Most from Your Lentiviral Transduction

Plasmids 101: Control Plasmids

Plasmids 101: The Promoter Region – Let's Go!

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Plasmids 101: Biotinylation

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