...This operon is under the transcriptional control of a strong constitutive promoter (BBa_J23100) and expresses...blue light.  Similarly, light can also be used to control homo-oligomerization of a protein fused to CRY2...

...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or.... Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand...breaks, a new DNA sequence can be knocked-in, or introduced, into the break sites. Knock-in experiments are...

...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

...find a title, author names and affiliations, introduction, methods, results, conclusions, and acknowledgements...flanked by two equally-sized columns, where the introduction, methods, conclusions, and acknowledgements ...the work. The other poster sections like the introduction, methods, and figures flank the central region...

...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... typically includes a couple of sentences of introduction to my area, a line about the research I will...you can find some great information on ELISA troubleshooting and multiplex assays.   Additional Resources...

...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...

... recombinase.   Once the SSR of choice is introduced, recombination can proceed either by first utilizing...alone rescues the phenotype being studied. Introduction of a conditional point mutation or functional...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

...replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019... a multitude of configurations can be used to introduce specific target modifications. Since this means...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...

...background without an introduction. If someone approaches your poster, introduce yourself and ask about...have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I walk up ...

...pitch,” the 30 - 60 second blurb you will use to introduce yourself and what you do. You might want to include...research. Try to avoid jargon in this initial introduction. During the conference When you meet someone...is what their project is. Or maybe you want to introduce yourself to a scientist whose work you are familiar...

...selecting an advisor, it is always a good idea to introduce yourself to a few members of your desired department... potential PI should go something like this: Introduce yourself, brief educational background, and that... meetings can still be a great opportunity to introduce yourself and learn more about your schools of ...

...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

...evaluated what lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas ...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest for about...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

...complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods for repairing...NHEJ is error-prone, such a system is likely to introduce additional nucleotides flanking the insertion....the DNA ends anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition...

...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ...

...impossible for a career scientist such as Zimmer to introduce any one of these topics without some editorializing...fellow scientists--but that doesn’t make his introduction to these topics any less effective. Who is this...non-scientists in your life, the book may be the introduction they need to grasp the challenges you face in...

... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

...average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs. This library was designed to improve on-target...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

...preferred platform before you start can save a lot of trouble in the long run.  Selecting the method Once you... Requires regular content production. Internet "trolls"   More resources on the Addgene blog:  Summer...  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...