We narrowed to 490 results for: Vit

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...

2. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   Published

   April 18, 2024, 1:15 p.m.

   ...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...

3. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ... receiving applications, universities typically invite a select group of students to continue the application...

4. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

7. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...to include several other important pathways. We invite feedback and contributions from all members of ...

8. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...

9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...groups. What apps do you use to improve your lab productivity? As this is a growing market, I am sure I missed...

10. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...releasing simply plug into this existing economic activity. People are using BioBrick parts to make more ...

12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...membranes and permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize...

13. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...

14. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...and rapidly caused the death of adenoid cells in vitro (Rowe et al., 1953). While overt death of the targeted...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms a novel co-complex with the...

16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... last decade. He describes the detection of gravitational waves--a story I was much less familiar with...

17. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...various mutations used to increase its stability activity, can be readily purified using plasmids found ...

18. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...

19. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging...

20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type

    Blog Post

    Published

    May 8, 2018, 1 p.m.

    ...open source and free for all non-profit uses. We invite you to try ICE today and let us know how it works...