We narrowed to 487 results for: Vit

1. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...

3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

   Type

   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...

5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...

6. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...groups. What apps do you use to improve your lab productivity? As this is a growing market, I am sure I missed...

7. Plasmids 101: Multicistronic Vectors

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   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8...

8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...releasing simply plug into this existing economic activity. People are using BioBrick parts to make more ...

9. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...and rapidly caused the death of adenoid cells in vitro (Rowe et al., 1953). While overt death of the targeted...

10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...membranes and permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize...

11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... last decade. He describes the detection of gravitational waves--a story I was much less familiar with...

12. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms a novel co-complex with the...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...various mutations used to increase its stability activity, can be readily purified using plasmids found ...

15. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...

16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ...Karmakar et al., 2024; Karmakar et al., 2025). We invite researchers to explore and use our TnpB-based genome...

17. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...

18. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...They demonstrated REVeRT in various contexts in vitro and in vivo, including in human retinal organoids...

19. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ...you stand out.  In some fields, a cover letter is vital, particularly in fields where communication and ...

20. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function....