We narrowed to 530 results for: abo.2

... the transcriptional level.     Fig. 2 Tetracycline on/off promoters and modes of action ...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

...closely mimicking the future application. In figure 2 we provide an example of the comparison of the practical... single amino acid. Without further information about properties like photostability or aggregation tendency...Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional Resources...

...cellular responses in addition to antibody response 2) very high immunogenicity 3) and long-lasting immune...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process.   About the author: Benedict Diederich received his PhD ...

...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...

... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

...higher quenching of mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...but be sure to let us know if you'd like to write about your plasmids in a future blog post. No time to ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...you plan to use GMAP for your experiments, think about depositing your new plasmids with Addgene to help...

...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources...

... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. ...

...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...

...with abundance whenever possible.      Fig 2: Try to inversely pair fluorophore strength with protein...fluorophore for an experiment is easy, but what about those times you need more than one?   Multiplex ...

...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for...to high copy numbers. This creates a potential metabolic burden on the yeast cells. Some phenotypes may...

...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

...beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr ...20th anniversary was about all the amazing years behind us, as much as it as about the amazing years in...Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In the beginning...Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016, we also... As the pandemic slowed, Addgene began to think about how we could expand our support for the research...part of the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative....

...Antibody Fragments. Antibodies (Basel). 2019 Apr 9;8(2):28. doi: 10.3390/antib8020028. PMID: 31544834; PMCID... time, when someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD...mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-crystalize...for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments...2019). Structural diversity and flexibility of diabodies. Methods. 10.1016/j.ymeth.2018.09.005 Muyldermans...

...the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn is a...include a note about where you met IRL (in real life) and perhaps what you talked about (or how good the... this rule, but not that many). I’m asked a lot about requesting connections to complete strangers for...have been able to tell when some start-ups were about to be bought by seeing certain groups of people ...using social media as a scientists Read blog posts about networking ...

...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into... blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about the differences ...library were designed using structural information about parental channelrhodopsins to create recombinant...

...main decisions to make: 1. Which modification? and 2. Which viral vector should be used? Modifying gene...general consideration for behavioral testing in a laboratory, a high level of experimental control is required... after stereotaxic injection. Find more details about choosing AAV or lentiviral vectors in our previous...it’s crucial to appropriately time the injection about two weeks before the behavior test. This time window... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...