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...domain.   Check out our new Isotype trading cards!     References and resources References Janeway...

...obvious component of a biosensor is the thing that you care about within your cellular or animal system. This...

...of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus on antibodies...

...a transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic...

... through Addgene. These plasmids can be used to carry out any biochemical and biophysical study involving...

...keep track of what works, and what does not work. Careful documentation will keep you on task and will prevent...

...result in a high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible...

...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...

...laboratory animals during in vivo research and the CARE guidelines for case reports are two sets of guidelines...

... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

...Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements...

...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...

...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April...

...Epitope availability References Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, ...

...proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary...

...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...

... be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for integration at a...

...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried...

...different plasmid backbones that varied in size or carried different genes. In these cases, gene expression...