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...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

...library allows a researcher to label individual members of a starting population of cells with specifc,...

...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...