We narrowed to 517 results for: emb

1. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

2. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

3. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

4. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

6. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

7. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

8. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

9. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

11. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...library allows a researcher to label individual members of a starting population of cells with specifc,...

13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

14. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

15. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

16. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

17. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

18. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

20. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...