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We narrowed to 811 results for: lars

Showing: 421 - 440 of 811 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...avenues for therapeutics that treat disease at the molecular level. For instance, if a disease is caused by...vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery vehicles...hemophilia B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable...on gene therapy for viral infections. She is particularly passionate about HIV cure research and science...

  2. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...skills Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association...provides a peak into their day-to-day business with popular work experiences of 3-10 days. And the Society ... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

  3. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, ...Viruses have become a regular part of basic biological research as well as clinical therapy. These biological...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  4. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... your interview The STAR interview method is a popular way to answer behavioral interview questions. STAR...key decision. Even deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...need to design and optimize a pegRNA for your particular edit. These tips are a great starting point: ...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...and resources page Browse our molecular biology protocols Read our molecular biology reference ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...worm so check back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN...

  7. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... several reasons. Casɸ is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...edit due to toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature...with neuron bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...bench scientist completing my graduate studies in molecular biology and epigenetics. So, I sought out a number...be understood by those who are not experts in particular scientific fields. As scientists, many of us ...Find all of our science communication posts Extracurricular activities for a strong science career path...

  11. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... close to the hood so you can view your cells regularly (both before entering the hood and when putting...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies... the secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

  12. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...Their in Vivo Persistence after Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Recombinant Adeno-Associated Viruses. In: Methods in Molecular Biology. Humana Press, pp 247–278 Additional resources...

  13. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...long as the disciplines have existed, cell and molecular biologists have been continuously pursuing new...Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still quite small... become one of the most commonly used tags in molecular biology. And the Myc1-9E10 antibody remains a ...experiments. It turns out that even with the most popular antibodies, context is key.    References and...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... generate rAAVs in your own lab with standard molecular biology tools and tissue culture experience. Here...Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580...cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org...B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T., Darmon, C., Doria, M., Douar...

  15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...isolated, and disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers...existing bacterial CRISPRi methods. First, its modular design allows for customization with strain-specific...generations. Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with...be used in non-model bacteria species where its modular nature allows for the customization of guides, ...

  16. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... applied for jobs, I knew I should negotiate my salary after receiving an offer but even as I did (successfully... wearing business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The...prioritize in a lab with both electrophysiologists and molecular biologists? What is their perception of me and...working with you will benefit them too. Ed's note: Salary negotiations will be covered separately in a future...

  17. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the use of degron tags has become increasingly popular for the modulation of endogenously and exogenously...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...and I guarantee you have developed them through similar experiences in graduate school. These skills will...they use, you’ve gained experience working with similar protocols, and look - they’re seeking someone who... be assured that you will contribute openness, clarity, approachability, and professionalism to team meetings...strength until it is identified by another person. Similar reasoning may also apply to other acquired skills...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term which...mutagenesis (which HIV does not do). Furthermore, molecular biologists working with vectors are, in most cases...on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References...synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183. https://doi.org/10.1016...

  20. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...be good targets for therapeutic development, particularly in cancers that are more common in individuals...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...confounding factor when interpreting findings, particularly when researching ancestry groups. And, of course...
Showing: 421 - 440 of 811 results