We narrowed to 714 results for: mak

...off of a circular plasmid via PCR. Typically, to make these constructs, developmental biologists have ...please write to [email protected] and if you make new plasmids for investigating different planarian...

.... The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project because...PubMed Central PMCID PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K.,...

...effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then the treatment will...systems as well as improving delivery strategies to make fast, effective, and targeted therapy possible. ...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...receiver cells. There are over 40 trillion cells that make up the human body and G-baToN provides versibility...

...When it comes to making the genetic manipulation, there are two main decisions to make: 1. Which modification...

... of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — ...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...

...to CRISPR experiments, therefore, you often must make a choice about what is your top priority: location...trying to knock-in a patient mutation or need to make a precise amino acid change, there is very little...

...safety profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are...homology directed repair (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated...

...low-cost, field-based diagnostics, our approach could make an impact. Our motivation was underscored by the... switches and amplification primers. This helped make sure that we had a number of successful sensors ...

...cell membrane. His recent work shows that they are making fairly remarkable progress on establishing a system...the drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These...

... humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...purified. Although a ~1% change in signal might not make a very powerful tool, it was promising enough to...

...additional datasets used in the training of PRIDICT2.0 make this model more suited for prediction of very diverse...includes applying machine learning techniques to make sense of biology, with a special focus on gene editing...

...chromatin interactions, they are grainy so it’s hard to make out the details. To increase the resolution, the...genomic loci of interest. This is cumbersome and also makes it hard to compare results across multiple targets...

...chromoprotein detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if ...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising.... 1997; Schiweck, et al. 1997) with the goal of making the antibody more amenable to protein engineering...

...separates the two components of the indicator, which can make the kinetics of voltage sensing to signal output... (voltage sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for ...

... inadequate plasmid DNA yields. Why would volume make a difference? In the simplest of terms, more volume...cells present in the given volume.  Let oxygen in! Make sure your culture is getting the right amount of...

... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...and mCherry). These simple strategies can help you make better fluorescent fusion protein reporters for ...

... learned along the way and hope they’ll help you make the most of the opportunities you’re provided throughout...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest...

...made publicly available through Addgene.  What makes CRISPR so special? A major advantage of CRISPR/Cas9...  2) What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout...