Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 855 results for: ple

Showing: 421 - 440 of 855 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To...do so with fluorescent protein labels requires multiple fluorescent proteins whose excitation and emission... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...

  2. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... with your CV to people you’d like to work with next.” Connie Cepko looks for people who are dedicated... and interested in science. “Postdoc hires are simpler because of their documented history in science....satisfaction (especially for scientists and technical people) is linked to engagement and access to growth opportunities...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...neuroscience experiments often rely on multiplexing, where multiple fluorescent tools are used simultaneously...can image multiple brain regions or cell types at once using existing indicators. For example, you might...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...

  4. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...and even allowing multiple proteins to be visualized simultaneously. Complementation systems for examining...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...present in the system at a given point in time. For example, if ATP was the molecule of interest (analyte),...depending on the biology you are studying. For example, calcium dynamics in the cytosol and sarcoplasmic... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...all have specific sensors designed for them. For example, this class includes tension monitors associated...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ...applications these viruses are good for, we have plenty of information on that too. May your titer always...

  8. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...applications, while electroporation of a human, for example, isn’t a reasonable option. Since viruses used...used in labs are derived from viruses that infect people in nature, extra safety measures, like replication...therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580... Multiple Authors...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis?  A: Basic science and preclinical researchers...

  10. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... For example, setting up your pipette tips to match your plate layout can help you track samples as you...change variables to fix a failed experiment.  For example, I used to constantly underestimate time needed... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

  11. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...help my research and my career. Here are three examples of how using social media has helped me or my ... can start using Twitter and provide real-life examples of how they and others have used Twitter professionally...also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what...do you do? — Stephani Page, PhD 👩🏾‍🔬💜 (@ThePurplePage) February 13, 2014 2. "The Scientist's Top... for Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    .... This luminescent technology is based on the principle of bioluminescence resonance energy transfer (...photobleaching/phototoxicity, they successfully imaged multiple subcellular compartments over several minutes ...improve long-term imaging, which is limited by depletion of luminescent substrate, they synthesized diacetyl... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...

  13. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other... we definitely don’t have that you really want, please let us know! And if you study chromatin and want...t see the gene you’re looking for on this list, please consider searching directly on our website. ...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...not the first Addgene blog post about chromatin--multiple Addgene colleagues and guest bloggers have written...

  14. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... Methylases involved with these processes (for example Dam and Dcm methylases) are independent from the...conversely, activating enzymes such as DpnI. For example, cleavage by XbaI may be blocked due to methylation...those sites will be methylated. Using the same example plasmid as above (which has the XbaI site blocked...methylation by altering your choice of bacteria. For example, if you must use a restriction site that will be...produce DNA that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an...

  15. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...labels, since I often work on multiple projects in a given day. For example, instead of naming a file “output...experiments can be found in each folder. This can be a simple .txt document. Record the name, date, and location...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...result was, so try not to do this. These are the simple rules that I try to follow in my own research. ...

  16. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...outlined in Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time...calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours, so at least...published in. That’s also a great way to get to know people doing interesting and relevant work in your field...That way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you... Multiple Authors...

  17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest... the other Cas family members make for useful implements in the scientist toolbox. If your genome engineering...

  18. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills...problem.  Step Three: Decide on a strategy and implement it.  Step Four: Assess how effective your strategy...the cause. To test the most likely cause, you implement an experiment designed to fix the problem. Then...modifications, and ultimately decided on the changes to implement. As with any skill, there will always be room ...Know your strengths! Research experience presents ample opportunities to practice problem solving skills...

  19. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...of the sample due to the smaller size of RANbodies compared to antibodies. Staining with multiple HRP-RANbodies...used at a time. Samples can be sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies...process that’s difficult for individual labs to complete. The nanobody based RANbody platform from the ...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...system contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of...interest.   CcdB resistant E. coli strains complete the system But ccdB alone isn’t the only key to...most appropriate method for your own experiments. Please check out Addgene’s Choosing a Molecular Cloning...protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed...
Showing: 421 - 440 of 855 results