...gene is expressed in a particular cell type is important not only for translational research, such as gene...expression of the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP ...expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where...

...New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab, high school...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...vectors are available? What you’re building is important — but so is where you’re building it! Table 1 ...

...sites within the viral genome to excise a large portion of it. This ongoing trial is sponsored by Excision...effectiveness, and delivery. Patient safety is important for allogenic therapies to prevent a graft vs....vs. host response. Off-target effects are also important safety considerations for all CRISPR therapies...

... would be infected by a lab-grown virus, it's important to understand the risks of the specific virus ...waste, remember that the percentage indicates the proportion of the decontaminate in the waste itself, not...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...

...conclusions. Being vigilant about these errors is an important part of maintaining the quality of reagents, especially... especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are ...plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing. This platform sequences the entire...

...that infect bacteria), many of which are doing important work in our microbiomes. Given that viruses are... and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze thaw ...vector: optimization of conditions for storage, transport and delivery." Japanese journal of cancer research...

...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...

...still working at the bench. It was a fantastic opportunity to talk to so many scientists who were potential...field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was presented... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

...combs. To make genetic crosses properly, it is important to isolate females from males while they are still... after hatching into their adult phase. It is important to clear out the parent crosses from the container...feature when setting up genetic crosses. More importantly, this knowledge will stop you from embarking ...

...experiments where maintaining library diversity is important, while lower transfer rates are only suited for...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown in presence of trimethoprim...

.... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...frequent collaborator and is happy to give advice and support for follow-up studies. “We don’t have a vision ...

...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-...the likelihood that donor DNA will interact with sporadic double-stranded chromosomal DNA breaks “that always...

...all the components of the biosensor in optimal proportions from a vector backbone that allows lentiviral...Waldron We recently added two antibodies against important targets for neurodegeneration research: Anti-MAPT...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates..., CRISPR may increase this rate. Specifically, portions of the AAV genome can integrate at double-stranded...

...post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional...strong evidence that a particular gene is actually important for a given phenotype. Each hit from the screen...

... These parts have been carefully engineered to support reliable functional composition. In other words...YouTube primer on BIL gates.) Addgene: Why is it so important to you that these parts be made freely available...language is put into practice? Endy: I think it's important to understand that these platforms, or pieces ...

...conditions when compared to FRET. However, it’s important to note that BiFC is irreversible — FP fragments...interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many...equal parts neuroscientist, writer, and extreme sports enthusiast. He recently obtained his Ph.D. in neuroscience...

...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

...for further processing. It may be helpful to incorporate regular feeds during this incubation time to ...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...