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Showing: 421 - 440 of 635 results

  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...experiments with/for them. Though not the most prestigious role in a company, Technical Support is essential... open to experienced professionals with strong interest in the technical aspects of science and the drive...

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...understandable to scientists outside your field, and interesting. For example, “there is a significant need for...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Let's Go! How to Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid...Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest Analysis We also introduced guides...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... answers to such questions, Addgene is deeply interested in pursuing them. We’ve recently revamped our...can help others as much as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 copies of the short epitope...portion of the receptors recognizes an antigen of interest while the intracellular T cell receptor portion...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40% after G418...designing homology arms with the modification of interest, ordering them from a gene synthesis company such...

  9. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...favorite Cas enzyme, a guide RNA to your site of interest, and a donor DNA molecule containing your desired... these numbers may vary based on your locus of interest and experimental system.  Synthesizing ssODNs ...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...standard curve with a pure sample of your protein of interest (POI). This approach is reliable, but it’s overkill...BioRender.com. On this gel, the protein of interest band is significantly lighter in “condition 1”...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...every lab, there are certain hierarchies and restrictions to data access. If these are important in your...Pavlek is a Product Marketing Manager at SciNote, interested in combining scientific research with tech advances...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...between a pir-containing donor and the recipient of interest. Because pDS132 cannot be replicated in the recipient...Depending on the growth rate of the organism of interest, from the day primers arrive, the desired chromosomal...

  13. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...trainees at low wages will still be there without restructuring. Positive Points for Trainees - Taking Steps...of Research Website Additional Publications of Interest Can Be Found Here Resources at Addgene  Read...

  14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...development of the corneal epithelium. For scientists interested in neuroscience and development, Brainbow is ...organization of the brain and other biological systems. Interested in applying Brainbow to your research? Check ...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...and repair pathways work downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert...Validated gRNAs to Target Your Genomic Locus of Interest Browse the CRISPR Collection ...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either...Voltage Sensor  Ingaki and colleagues (8) were interested in using photoactivatable, optogenetic accuators...

  17. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding...novel base editor proteins. She is particularly interested in combining the realms of art and science. Follow...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...joining for recombinant plasmid construction (restriction cloning), adaptor ligation for next generation...(LDR), probes complementary to the sequence of interest are annealed to target DNA (genomic DNA or a PCR...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic....A., and Callaway, E.M. (2007). Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...
Showing: 421 - 440 of 635 results