We narrowed to 489 results for: vit;

1. PCR Applications for Copy Number Variation Assays

   Type

   Blog Post

   Published

   April 26, 2022, 1:15 p.m.

   ...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...

2. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

4. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

5. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

6. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...to include several other important pathways. We invite feedback and contributions from all members of ...

7. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...

8. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

   Type

   Blog Post

   Published

   Sept. 23, 2014, 3:05 p.m.

   ...groups. What apps do you use to improve your lab productivity? As this is a growing market, I am sure I missed...

9. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8...

10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...releasing simply plug into this existing economic activity. People are using BioBrick parts to make more ...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms a novel co-complex with the...

12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... last decade. He describes the detection of gravitational waves--a story I was much less familiar with...

13. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...and rapidly caused the death of adenoid cells in vitro (Rowe et al., 1953). While overt death of the targeted...

14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...membranes and permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize...

15. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...techniques involve the assembly of a circular plasmid in vitro, before transforming it into E. coli for propagation...

16. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...various mutations used to increase its stability activity, can be readily purified using plasmids found ...

17. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...

18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...mice strain specific Ly6a SNPs on PHP.B binding in vitro. They found that when LY6A contained C57BL/6J SNPs...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...regulatory regions that are necessary for myosin activity and thus interaction with actin in nonmuscle systems...

20. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ... seek to convey in your chalk talk, there's the vital task of engaging your audience. You need to make...