We narrowed to 783 results for: INA

...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited... more than 45 publications, beginning with the seminal research articles that demonstrated the feasibility... Each screen curated in ORCS is linked to the original publication via its PubMed identifier, which can...metadata.   Screen results are reported as the original quantitative gene-level scores provided by authors...rather than sgRNA-level sequence read scores. The original supplemental data tables available with the publication...

... the protein with enterokinase, which recognizes the 5 amino acids on the C terminal of the tag. Of the...background can be a huge challenge. Epitope tags eliminate the need for target-specific antibodies and have...genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you are fortunate...protein that can also be used as a tag! GFP was originally derived from jellyfish (Aequorea victoria) and...

...conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies are plasmid-based and ...The resulting chimeric antibody would have the original variable regions from the parental rat antibody...antibody retains the binding specificity to the original target and can be used with secondary antibodies...is routinely used to increase the utility of recombinant antibody tools, the resulting chimera may have...

... PMID: 30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein...Weinstein Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for...organism. The SPARC system uses competitive recombination sites to selectively remove a stop cassette ...effector is expressed. By truncating one of the recombination sites in the construct to different lengths,...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...

...by Klaus Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains...and as a consequence, EB1 protein function is inactivated by the separation of its synergistic domains....and colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and... editors that utilize dCas9 fused to cytidine deaminases like APOBEC. To achieve this they created a lentiviral...efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify...

...single-cell sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring. ...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ... Christina Mork...

...In our case, we will add TAAGCA, resulting in a final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC...amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop codon (5...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA...restriction cloning, it is essential that you verify your final plasmid by sanger sequencing. DNA replication by... you use, it is important that you sequence the final product. Additional Resources on the Addgene Blog...

... your protein of interest will only occur in combination with that reporter, you can infer its presence...using a mix of different antibody-fluorophore combinations for different markers of interest, you can separately...for distinguishing live from dead cells. The combination of antibody-fluorophores, fluorescent reporters...BV421 and RB545. Fluorophores can also come in combination of two conjugated single fluorophores, like APC-Cy7...be emitted by either of the two. As a result, inaccuracies in the detection of fluorophores (and the markers...should try to avoid or reduce it whenever possible. Finally, we designed a simple panel to analyze transduced...

...Gautier et al., 2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity...(Erdmann et al., 2019). Check out our recent Illuminating Choices blog post for more discussion about ...Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D. K... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana...from Addgene Better Dyeing Through Chemistry Illuminating Choices: A Guide to Selecting Fluorescent Dyes...

...demonstrated in different cellular environments such as retina or glioblastoma). They also found that exo-AAVs... protects them from antibody recognition and inactivation (György et al., 2014), but scientists have yet...deafness (György et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar...difficult to reach, and are more resistant to inactivation through anti-AAV antibodies. References György...vector mediates robust gene delivery into the murine retina upon intravitreal injection." Scientific reports...