Skip to main content

We narrowed to 611 results for: STO;

Showing: 221 - 230 of 611 results
  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...
  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...like the cold or flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics...those bacteria that are partially resistant. If you stop a treatment early, you may be un-wittingly selecting...used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem...
  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...modification with fatty acyl chains. For instance, myristoylation requires an N-terminal consensus sequence (...variants and check the functionality of the fusion (Mastop, 2018). Summary GFP is an all-round useful genetically...21619590. PubMed Central PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring...
  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...
  5. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ... clinical application in under a decade is an astounding feat of public investment and collaborative scientific...effective treatment. And base editing advances won’t stop here. By coincidence, KJ’s genetic sequence was ...., Correia, B. E., … Kleinstiver, B. P. (2025). Custom CRISPR-Cas9 PAM variants via scalable engineering...
  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...integrin antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim...
  7. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...a room, but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote...control some basic functions, including starting and stopping recording).  Test: It was unbelievable how well...the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need...
  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...s a guide that directs cleavage right before the stop codon!  Figure 3: How to design a C-terminal...guide would be selected, since it cleaves at the stop codon TAG. Design your repair template with ...
  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...2006): 703-9. PubMed PMID: 16582903. Potter, Christopher J., et al. “The Q system: a repressible binary...from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling...s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a functional...
  10. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...site. Recipient cells express DsRed within a lox-STOP-lox sequence, followed by the EGFP, and the TEV ...translocates to the nuclease, and excises the DsRed-STOP to trigger expression of EGFP. Figure 2:... MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023). The power and potential of mitochondria...
Showing: 221 - 230 of 611 results