We narrowed to 884 results for: ica

...the OpenWorm website. microPublication microPublication is a unique publication for the worm community...into larger publications and would otherwise be lost in lab notebooks forever. microPublications are peer-reviewed...aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important ...Biodesign institute developed a 3’UTR database specifically for C. elegans. This database provides detailed...normally one figure and can be negative and/or even replicated studies to show reproducible results. This a ...referenced. In addition the data published in microPublications is curated and added to other databases including...including wormbase and flybase. microPublication is run and maintained by members of the worm community...

... do anything. Viruses are complicated and they perform complex biological tasks, both of which are factors...C virus RNA quantification as measured with branched-DNA technology." Journal of clinical microbiology...factors in the technical challenges they can present. Many researchers find themselves plagued by troubleshooting...hail Marys that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors...vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...Different titering methods can reveal differences in physical versus functional titer. If you have low titers...cells, replace the medium with half the volume typically used (e.g., use 5 mL medium on a 10 cm plate)....

...experiments. However, the technical demands of using these wireless devices are significantly higher than standard...optic cable is threaded through—and fixed to—an optical insulator called a ferrule (below). The fiber optic...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...There are several important considerations with fabrication of the fiber optics needed for optogenetics. ...point; the fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency...maintained during the entire protocol, otherwise optical stimulation will be erratic, opsin activation/inactivation...stimulation, the rate of movement of the animal significantly increases. Before you get your experiment started...

... editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https://doi.org/10.1093/abbs/gmab036...for successful modifications, efficiency can be low, especially for larger modifications. What’s the solution...tools available for bacteria and some of the applications for which they’ve been used. The beginnings ... of Enterobacteriaceae, without the need for modification. This finding suggests that the system may be...eukaryotes (Qi et al., 2013). As in other systems, catalytically dead (dCas9) targeted to a promoter or gene ...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or...responsive promoter to drive expression of a catalytically dead Cas9 enzyme allows reversible repression...

...Castano Corsino, a junior faculty member at Harvard Medical School/Brigham and Women’s Hospital (BWH). Based...like IMP, bring closer than ever (although not physically) the ideas and experience from talented professionals... IMP USA-Europe not only provide the specific technical and logistic support to increase the chances of...professional opportunities that are outside of our typical boundaries, and will arrive to Spain at one point...international mentors. Universities from five different geographical locations in Spain were enrolled: the University...represented a diverse group of individuals with technical and career path needs, and came from the universities...that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...

...biosensor for cAMP dynamics. The mGold2 variants significantly enhanced photostability — 25-fold over mVenus...between mGold2s and mGold2t depending on the application. Figure 1: Super-resolution imaging...fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification of photostability. Image reused from Lee & ...fluorescent proteins for extended imaging. Nature Communications, 16(1), 3241. https://doi.org/10.1038/s41467...proof-of-concept, they believe that the strategy will be applicable with other TnpBs and in other plant species, ...available for tracking cell lineage, different applications require careful selection or design to ensure...for gene therapy and ex vivo cell engineering applications and is especially well-suited for lung cells...

...lab while the publication page contains all the plasmids deposited from the same publication. Next up, we... Addgene’s plasmids is an indication of popularity. A yellow flame indicates that a plasmid has been ordered...through the depositing lab page, then onto the publication page, and finally on down to the individual plasmid...Great! Now, if I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing...plasmids that are available through Addgene. Publication: If the plasmid has been published, the paper...the bottom of the page that help you cite this publication in your own work: How to cite this plasmid: The...depositing lab whenever their plasmids are used in a publication. Addgene makes this easy by providing a template...

...methods. Since molecular, ecological, and reproductive confinement typically require very little effort...Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms ...the entire wild population with unpredictable ecological effects. Because the consequences of such a mistake... to implement and have been tested in yeast5. Ecological confinement involves performing experiments in...experiments attempting to build gene drives in tropical mosquito vectors of diseases such as malaria and...they should not be relied on exclusively because historical studies of pathogen research have conclusively...strategies is strongly advised. The effects are multiplicative, and there are very few gene drive experiments...

...their undomesticated counterparts) - not exactly species that jump to mind when we think “biomedical research...ways these animals have impacted humans through biomedical research. The Camelid family is composed of ...research". The family entered the biomedical field’s spotlight after researchers discovered heavy-chain...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative...chromobodies,” which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize...therapeutic was approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics... excitement around using sdAbs in diagnostic applications. For example, fluorescently and radioactively...

...origin of replication (which controls the host range and copy number of the plasmid) and typically include...Description Origin of Replication (ORI) DNA sequence which allows initiation of replication within a plasmid...point for PCR amplification or sequencing. Primers can be exploited for sequence verification of plasmids...plasmids are small circular pieces of DNA that replicate independently from the host's chromosomal DNA....Minimally, lab-created plasmids have an origin of replication, selection marker, and cloning site. The ease... plasmids and the ability of plasmids to self-replicate within a cell make them attractive tools for the...plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for selection of plasmid-containing...