We narrowed to 868 results for: REN

...available when they are validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease... system, see Yamamoto and Sakuma’s protocol. Reference: Multiplex genome engineering in human cells using...

...letter abbreviation of the mutated amino acid currently at that position. In contrast, unmutated or Wild...  Matthew Ferenc...

...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...

...detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific discourse...made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing what they call “...

...virus works its way into immune cells in ways that aren’t fully understood. Much of the action depends on...relevant scientific literature.  -In light of the current Ebola crisis, Science and Science Translational...

...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...training programs. I believe in the academic apprenticeship system as a training ground for scientists,...

... buyer to a seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does...you listed during checkout. This invoice will reference your Addgene order number and PO number that you...

... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

...Research currently under way in the Kotton Lab. One of Darrell's favorite projects - differentiating surfactant...

...hisashii, BhCas12b that is functional at 37 °C but preferentially nicks the non-target DNA strand. The lab then...approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-Cas12a proteins...

...or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ...

...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende...

... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...

...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes...

...never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because of this.... And don’t forget to follow us @Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ...

...strives to match up scientists across fields with different technical knowledge that could help fight COVID... both clinical care and public health for this current COVID-19 pandemic, and outbreaks that have yet ...

...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete...

...the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ...

...Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity...

...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...

...meiosis in biology. Lots of it is researching the different aspects of the subject you're writing and then...developing new online courses about unique topics that aren’t already offered by eScience Labs.  “We work with...

... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...

...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...

...includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...

...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science...

...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...

...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...