We narrowed to 534 results for: CAR

1. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...mushy, gooey contents of said containers, think carefully about how you want your software to treat those...

2. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...distribute through our deposit process, and then we carefully track samples through our lab using barcoded tubes...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   .... PubMed Central PMCID: PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-dependent target...

4. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...like papers that can end with some sort of model (cartoons are appreciated) to visually sum up all of the...

5. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional...

6. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...

7. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements...

8. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette...

9. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged...

10. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies...

11. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...cells. What’s more, G-baToN can be used to bring cargo, such as protein or DNA, to manipulate function ...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...generally less common and will stand out; however, be careful not to hang your poster too low. If it is pinned...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ... - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences...

14. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...for your genes of interest, your F1 mice will be carriers at those alleles. Some alleles, such as Cre, function...

15. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ... developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA...

16. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...the virus so that it only enters and delivers it cargo to certain cell types.  Botond Roska’s lab at the...

17. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ... active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting, unlike other well-characterized...

18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... to make sure the deposit agreement/MTA is taken care of and, once we have all of your plasmid data, we...

19. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...improved transcript life, and so should be handled with care (4). For this reason, viral vectors often employ...

20. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...resolution of positive and negative populations, Careful panel design In both conventional and spectral ...