We narrowed to 553 results for: ESC

1. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

2. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...

3. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

4. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

5. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

6. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

7. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

8. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ... the exact nature of your mutations. A great description of the plethora of available sequencing options...

9. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...worries, I've summarized the three strategies described in this post in the graphic below - including ...

11. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

12. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

13. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

14. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein degradation...

15. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...Addgene’s blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...

16. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...

17. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,...

18. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ... interactions in events that occur on a short timescale, such as during drug action or cell signaling....

19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...candidate genes in TS iPSCs to identify whether any rescue cell migration and contraction defects. The lab...

20. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...to receive deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in...