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We narrowed to 680 results for: Eng;

Showing: 441 - 460 of 680 results

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

  3. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

  7. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...scientists of all types to stay up to date and engaged with new advances in published science. They also...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...viruses, with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of...2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point mutations...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): ...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...

  14. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

  15. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ... less time consuming and/or less technically challenging. Alongside there is a second-line team that takes...are just so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details...

  16. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    .... During a transient leaf transformation, the engineered Agrobacterium strain is brought into contact ..., 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...response to requirements that emerged from intensive engagement with a wide range of people at the University... The two guiding principles emerging from the engagement with the Wisconsin research community were to...

  19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable marker for unmarked...
Showing: 441 - 460 of 680 results