We narrowed to 530 results for: Erf

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...

2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...PMCID: PMC4777285. Further Reading Microscopy U. Generfal Considerations when Using Fluorescent Proteins...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...isolate particular organelles and their contents, or perform in situ hybridization experiments. In many cases...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...found in the culture media, you should consider performing PEG precipitation. Instead of sonicating the ...

5. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...

6. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...

7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...starving. I think plant biology is massively underfunded considering how important it is. I would really...

8. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ...it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at...

9. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...should be free to use and used for good. It's wonderful to have Addgene as a professional public-benefit...

10. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...trendline. R2 ranges from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ... PCR/sequencing While CRISPR-transposons are powerful and simple to use, users should keep aware of ...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...

13. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    Published

    July 9, 2019, 12:52 p.m.

    ...students each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others...

14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ..., Diaz MFP, Yong WH (2018) An Introduction to Performing Immunofluorescence Staining. Methods in Molecular...

15. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ... the cell’s nucleus. But since nothing’s ever perfect, neither is AAV. Its main limitation is the small...

16. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...

17. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ...increase HDR frequency by committing a break to performing HR before other factors were loaded onto the ...

18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors for neurons,...

19. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...of fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple...

20. Uncovering Ancestral Bias in CRISPR Research

    Type

    Blog Post

    Published

    Nov. 29, 2022, 2:15 p.m.

    ... screens they analyzed were designed to have a perfect match to the reference genome – even single mismatches...