We narrowed to 450 results for: HA tag
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TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....the bench waiting for you, prep for research work has to be done by the researchers themselves. Be ...rotation students, joining a new lab at any career stage, learning a new technique while visiting a lab, ...
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An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....There are many moving parts to our software, which has grown with the company. It would be easy to assign...changing needs of the teams at Addgene. Another advantage of Scrum is that it provides transparency to everyone... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...that retain the ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long... Tabebordbar et al. Long et al. show that the percentage of dystrophin-expressing muscle cells increases...delivery must be optimized to: 1. Reach a high percentage of muscle cells throughout the body, especially... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...temperature-sensitive materials, we noticed a large percentage of these shipments arrived in India at room temperature... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...genome-wide screens. Figure 1: Summary of the advantages of the new approach to genome-wide CRISPR screen... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...sequence and other regions of the genome. The main advantage of NGS is that it allows you to simultaneously... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...itself, like laser type and power, acquisition voltage, the concentration of the fluorophore, and whether...bd-spectrum-viewer. But what is the real-life advantage of a brighter fluorophore? Strong brightness makes... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....ever thanks to the virtual world that the pandemic has foisted upon us. Action Items: A simple place to...an account and start engaging with the #scipol hashtag. Often there will be posts about virtual meet ups...