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We narrowed to 771 results for: INA

Showing: 441 - 460 of 771 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Aqua Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian...pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene...pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian Express a gene...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis...mesothelioma tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the ...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...

  3. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely...develop over time. It isn’t feasible to completely eliminate the development of resistance; it’s a natural .... "Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly important...find my tips for a perfect science talk here). Finally, my main content organizer is the amazing app Evernote...

  5. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...marketing, commercialization, collaborative, and financial functions associated with the business. Commercial...business, in healthcare economics, or in some combination of these fields. I got my PhD a couple of years...trends and workings of the healthcare industry. Finally, on-campus resources like career offices, classes...

  6. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...mice is likely due to how the Deverman and the Gradinaru labs engineered PHP.B. PHP.B was created by first...suggested there was a single gene that had two codominant versions or alleles that was driving the PHP.... available from Addgene. References Holmes, Christina, and William L. Stanford. "Concise review: stem...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to induce gene...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...strongly supported their initial findings.  The team originally suspected that this new Cas7-11 might be phylogenetically...flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating as they are good at...Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. Zepeda, Kevin Holden, Jonathan S. ...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Addgene, you’ll usually receive an aliquot from the original library constructed by the depositing lab. (For... library should be very similar to that of the original library. Like amplification, this step also depends...concentration.  Screening time Phew! You are now (finally!) ready to use your transduced cells to perform...

  11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired... to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes...JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved...

  12. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology...sequences each week. Our internal software was originally designed to help us work in large formats, such...continuous updates. The generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary...

  14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...Created with BioRender.com. In recent years, deaminase-mediated base editing (cytosine base editor or...FJ, Fan C, Landry MP (2019) DNA nanostructures coordinate gene silencing in mature plants. Proc Natl Acad...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration...have been developed with different mechanisms to inactivate the integrase.  The IDLV life cycle When IDLVs... can be generated through either homologous recombination or non-homologous end joining (see Figure 2)...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...blotting, a primary epitope-targeting antibody can originate from a range of species; for example, rabbit or...include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin ligases; and many ...  More resources on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide...

  17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...sequence, people often use terminology like “C-terminal fusion,” but I find this very confusing because... into the POI can work well since the N- and C-terminal residues of the fluorescent protein are relatively...assess whether the chimera is still functional. Final words on fluorescent protein fusions There are several...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...you realize whether or not you have a natural inclination for this skill that you could strengthen. The...substantive (“Person C told Person A one thing and inadvertently told Person B something else.") The majority...through multiple channels is not reasonable or sustainable. That said, when you know that a person or team...

  19. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...from the B cell antibody genes from diseased or vaccinated humans. How do you produce scFvs? While it’s ...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...to have a check-in with my boss Jennifer. I had finally found my footing with the company. I’d identified... Bench series about PPE! We made this with a combination of in-lab filming and remote filming. Learn: ...ensure smooth filming in the future, but we were finally refining the process down to a place I could be...
Showing: 441 - 460 of 771 results