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We narrowed to 792 results for: LAT

Showing: 441 - 460 of 792 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Adenoid Cystic Carcinoma, Up-Regulation of pVHL along with Down-Regulation of HIF-1α by NDRG2 Expression...a cell line even decades after it is initially isolated. Regrettably this is not usually the case if you...official biorepositories or have been directly isolated from patients. In these cases, a test known as...

  2. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Possible roadblocks only become obvious at rather late stages (i.e. postdoc level) and can include the ...non-academic career options require additional study for late career scientists to become more appealing on the.... The issue was with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech...signals are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance the ...

  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...donor site; SAS, splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023...therapies. REVeRT supports advanced gene expression regulation, enhancing both the mechanisms of delivery and...

  4. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...it helps you later on to remember where you read particular studies or experiments. Later, you can easily...have to worry about having to hunt down sources later on.  A comment about writing: initially I felt that... all the proteins in the class that showed the relative sizes and the conserved domains. This information...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E variant ...bacterial species Many more Cas9 orthologs have been isolated, and our understanding of their PAMs is shown ...same species. Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue... an intrinsically high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059...

  6. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...to be translated into Modern Greek to be easily accessible today. Roughly speaking, we translated our software...sprints is themed and the stories in that sprint relate to the theme. For example, we could have a shipping...feature to work. We will then go back to this area later and tidy it up. Conclusion While none of us are...

  7. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected into the...general, technical replicates can be thought of as "plate controls". They are NOT independent and are typically...transfection of multiple separate wells (within the same plate) with purified plasmid from the same aliquot/preparation... the same source and were incubated on the same plate at the same time. Although the replicate control...

  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... design and engineering of fusion proteins is relatively straightforward. However, generating a fusion...sites or motifs that are necessary for post-translational modifications. Despite these potential issues...fluorescent protein. Thus, scientist began using relatively long linkers between the protein of interest ...C-terminal residues of the fluorescent protein are relatively close together (figure 1). Where should the fluorescent...

  9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...color, allowing for the fate mapping of cell populations over time. The ability to track single cell dynamics... possible from Brainbow. Tracing clonal cell populations using the Confetti mouse For mammalian systems...not present at the same frequencies. The small population size of CFP++ cells permitted researchers to ... and Lgr5 identify two functionally distinct populations." Proceedings of the National Academy of Sciences...

  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...requires time and knowledge. In a recent article, we translated our knowledge of electronic lab notebooks into...ELN should allow you to collaborate within the platform and avoid countless emails. Important collaborative...permissions to different lab members to avoid any issues later on. Do you need external teams or partners to access...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...fractionation kit or a lysing protocol developed for isolating proteins from a specific compartment. If your ...blockers, you can run a comparison of different formulations instead of concentrations. Most labs use protein-based... For nitrocellose membranes, you can even give gelatin a shot.  Remember that protein-based blockers degrade...in an assay, meaning that your normalization calculations will not be correct, and you’ll need to adjust... Splice variants Check literature Posttranslational modifications Check literature Highly...

  12. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...harassment Isolation – including preventing access to opportunities, physical or social isolation, withholding... questions in the sections below. Mentoring relationships can help identify your priorities and needs,...regretted it when the same thing happened to her a year later. I hear too many science trainees get warnings and...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...certain region or calculate a colocalization factor to assess the spatial relationship between the targets...need more signal, another strategy is to use a biotinylated secondary antibody then label that with fluorescently-conjugated... other antibodies are disrupting the binding of later targets, the sample is being damaged by all the ...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...tools for neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene...and Mark A. Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene...

  15. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... day or a week planning an experiment, even a relatively simple experiment. However, by taking this time...surprising findings when studying a seemingly unrelated problem. However, you have finite time and resources...background slides. If you don't have much new data, speculate on what you hope to accomplish and how your research...satisfying. Finally, many PFs are in committed relationships and/or have started a family. Those people are...

  16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...sequence but not the amino acid sequence of the translated protein.  These changes can be 5’ or 3’ of the...strains, expression of exo, beta and gam is tightly regulated by the endogenous phage promoter pL and repressor...for a mobile recombineering system, but tight regulation of expression is required for a successful experiment...endogenous red promoter pL and the cI857 repressor to regulate expression. An antibiotic can be used to select...

  17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...The machine is useful for distinguishing cell populations that have been tagged with different fluorescent...The optimization of GFP and its derivatives by the late Roger Tsien changed everything. For the first time...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering..."Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior." Science...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All that is required... UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid finds the...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...applications." Current Chemical Genomics and Translational Medicine 3.1 (2009). PubMed PMID: 20161833. ...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...hope that these perspectives, the optimism they encapsulate, and the solutions they promote help motivate...infertility or to argue against unnecessary clinic regulations, evidence-based research is vital in combating...Greenbaum Science and evidence-based policy and legislation hit home hard in Michigan, where programs like...worried that, because we were speaking out against legislators and because the march had been so well-publicized...

  20. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...LinkedIn. We are also exploring other social media platforms with active scientific communities and welcome...functionality and features that previously made the platform a mainstay of conversation in the scientific community...considering a gradual move off X as a response to major platform changes, such as limiting access and increased...
Showing: 441 - 460 of 792 results