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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 708 results for: MAK;

Showing: 441 - 460 of 708 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...are of no use to you. When using more than one FP, make sure their emission light does not overlap in wavelength...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... a specific field. While searching for jobs and making connections, avoid falling into an identity trap...yourself, and to demonstrate them to the people who make hiring decisions. Though having all the technical...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...has greatly enhanced the ability of scientists to make genomic alterations, bringing about a revolution...the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR applications where limited...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

  9. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

  10. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...excited because genome-editing with these molecules makes it much easier to knock-in LexA binding elements...developing several additional systems of enChIP to make the technology more user-friendly and precise. We...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...the list of antibiotic options doesn’t end there. Make sure you read the instructions for whatever antibiotic...kingdoms of life. A bit of careful design will help make your experiment with shuttle vectors a success! ...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

  14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...as well as high spatial and temporal resolution, making it ideal for visualizing gene expression in vivo...They also did not scatter ultrasound nonlinearly, making them difficult to distinguish from background tissue...

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

  17. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...
Showing: 441 - 460 of 708 results