We narrowed to 463 results for: MEA

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...titering IDLVs, there is no integrated piece to measure! Instead, two commonly employed methods for titering...

2. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...sites used in these vectors are of low abundance (meaning they are unlikely to be found in your gene of interest...

3. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...resistance marker from the parental plasmid provides a mean for selecting for transformants which have taken...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ... the percentage of cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell...

5. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   Published

   Oct. 2, 2018, 12:56 p.m.

   ...failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders...

6. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...activity or gene expression. Experimental output is measured and quantified. Created with BioRender.com. ...

7. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...will cleave DNA frequently enough to result in a smear of fragments that get smaller in size as a function...

8. Finding Your Perfect Job After University

   Type

   Blog Post

   Published

   Jan. 12, 2016, 3:30 p.m.

   ...citizen, I was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia...

9. Building Global Connections with the International Mentorship Program USA-EUROPE

   Type

   Blog Post

   Published

   Nov. 6, 2015, 3:30 p.m.

   ...should not be confined by birth country or financial means. Instead, talent should be realized through equal...

10. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    Published

    Oct. 9, 2018, 12:21 p.m.

    ...and your development. Taken together, all of this means that you need to introduce yourself to and talk ...

11. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...achieve exponential amplification. What does fidelity mean for a ligase? DNA ligases generally prefer fully...

12. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...plasmid amplification (Fischer, KB. 2019). This means that all DIO/FLEx AAV vectors will have some level...

13. Important Considerations When Using AAVs

    Type

    Blog Post

    Published

    June 13, 2017, 2:30 p.m.

    ...Modified from Aschauer et al. (2013) What does this mean to us? Well, an AAV user must determine which serotype...

14. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    Published

    Nov. 28, 2017, 2 p.m.

    ... all team members understand what roles they’re meant to fill and what their responsibilities are. That...

15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...as their selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of...

16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    Published

    Dec. 15, 2016, 3:57 p.m.

    ...the substrate with the target DNA sequence. This means cloning occurs in vivo, as compared to restriction...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...experiment and test them in parallel so you have a good measure of ICC assay specificity. We generally include ...

18. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...environments. These studies provide quantitative measurements of cellular motility, interactions, and response...

19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...while the efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous...

20. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ... makes choosing the right tool confusing and it means that there is no one-size-fits-all approach. You...