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We narrowed to 663 results for: Mal

Showing: 441 - 460 of 663 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C....

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...amount and type of information you want to convey. Small tidbits and fast/fun facts are easy and engaging... be oversaturated. Transition from academic to informal writing can difficult.  Photography Medium...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...answer to the space problem. Instead of imaging smaller, what if we just made the sample bigger? In ExM...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...parties, and the company at large, by polling and informal communications we made a decision to try out a...the other hand if the journal club occurs with a small technical team (single lab or team), a deep technical...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...properly, it is unstable and may only work for a small number of qPCR runs before a new one is needed. ...and the droplet generation oil are moved through small channels to create a water-in-oil emulsion containing...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells could repopulate... systems used in both papers are analogous to mammalian viral vectors in that the vectors are replication-deficient...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...entirely. The other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding...toxic for the cells and affects the labeling of small structures like neurites. Like epitope tags, low...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...without changing the function of the sequence, normally through the synonymous mutation of a codon. You...and more accurate, but they also work with very small volumes of reagents which lowers experimental costs...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...requiring rapid maturation and prolonged imaging with minimal background.   Find these C. elegans mScarlet plasmids...18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 kDa) and are...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-negative  Acinetobacter...campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1 ...

  16. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... are also great at infecting neurons and cause minimal inflammatory response, but are not taken up by ...References: Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...

  18. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... treat a bacterial infection with a lower than optimal drug concentration. Drug resistance is not a black...patient infection. Limiting the use of antibiotics in animal farming is already being recognized as an important...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...://doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-Cas9...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...
Showing: 441 - 460 of 663 results