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We narrowed to 729 results for: NSI;

Showing: 441 - 460 of 729 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...

  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...

  3. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

  4. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

  8. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...

  11. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...inefficiently that no industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning...for research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the...

  13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...; or by incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs...assembly is cost, as T4 polymerase is much less expensive than the enzymes required for Gibson assembly....

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

  17. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

  18. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

  19. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using...exploring these interactions, and can give valuable insight into DNA expression and protein interactions, including...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...resources throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a ...
Showing: 441 - 460 of 729 results