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We narrowed to 790 results for: Por

Showing: 441 - 460 of 790 results

  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...experiments where maintaining library diversity is important, while lower transfer rates are only suited for...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown in presence of trimethoprim...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional...strong evidence that a particular gene is actually important for a given phenotype. Each hit from the screen...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ... These parts have been carefully engineered to support reliable functional composition. In other words...YouTube primer on BIL gates.) Addgene: Why is it so important to you that these parts be made freely available...language is put into practice? Endy: I think it's important to understand that these platforms, or pieces ...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...for further processing. It may be helpful to incorporate regular feeds during this incubation time to ...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...

  8. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

  9. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...vectors not only available, but accessible. The supporting data and community engagement required to do ...lessons learned from this process have shaped and supported our growth and development. We’ve shared what ...

  12. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...Labor Thomas Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional...National Postdoctoral Association Institutional Policy Report 2014). However, ~30% set their minima lower. Furthermore...consensus of academic stakeholders. There was ample opportunity to accept these recommendations, and make gradual...

  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...in the field and as always, we appreciate the opportunity to share these invaluable reagents with the scientific...

  14. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...this post will, indeed, focus on bacteria, it is important to note that the term ‘antibiotic’ can also be...animal farming is already being recognized as an important step, as you can’t walk into any supermarket now...Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & ...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected.... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  16. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...recent lineage tracing approaches that led to important insights in developmental biology. Microscopy-...pathway regulated by one-eyed pinhead, incorrect proportions of otherwise normal cell types are produced, ...PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    .... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...astrocytes, and oligodendrocytes, provided that reporter genes specific to these cell types are used. The...

  18. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned, and...more neuroscience-focused tools in their Brain Map portal, and cell science-focused tools at the Allen Institute...expression from mouse to primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...sequences, which facilitate integration of this portion into the host genome. The packaging plasmid contains...more detailed description). Since the integrated portion does not contain any of the viral packaging genes...the resulting double-stranded DNA transgene is incorporated into a large complex of both viral and cellular...

  20. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...signals. For this, you’ll need to gate based on the proportion between H and A (or W and A) within the same ... just as fine (Figure 4). As H and A scale in proportion (while H and W do not; see Figure 2), single ...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can ...
Showing: 441 - 460 of 790 results