We narrowed to 485 results for: Ret;
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TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....knows that you can design experiments with ddPCR, interpret the results, and develop a successful, reproducible...
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Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....higher) of BFP and GFP producing cells. After FACS, return the cells to doxycycline-free media and expand ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....evidence of growth inhibition when the system was retargeted. This finding was surprising – in the most common... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....software from Python 2.7 to Python 3.5. Python 2.7 is retiring this year, which means there will be no further... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....promoters is commonly employed in lentiviral and retroviral constructs and we will discuss these in a future... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....promise it isn’t.) The intuitive, qualitative interpretation of this gel is misleading. We can see why on... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m....color for its entire lifetime and daughter cells retain the same color, allowing for the fate mapping of... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....Spinach, a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....by Wrenbeck et al but it’s likely that longer stretches of sequence could be used. However, a practical... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....PMCID: PMC3129320. 18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....information is consolidated to one place, easily retrievable, searchable and traceable. However, switching... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....of protein molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013):... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....drawback for therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....). E. coli as a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....academic research. Panelist Moon Duchin provided a concrete example of how underrepresented individuals are...