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TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....AAV as a tool for gene expression in mammals. Subscribe to Viral Vector Blog Posts Additional Resources...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about other CRISPR ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....worrisome for me since bioethics is an inherently subjective area. For CRISPR, I focused on the fact that,... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for the identification... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m.... from Addgene and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed ... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....kind of content are you sharing? Sometimes your subject matter will drive the kinds of communications you... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....and potency with phages Neither group observed substantial resistance to the CRISPR antimicrobials, and ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires the... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m..... It is important to note that the person who submitted the idea did not have to be in charge of implementing... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m....antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog PostPublishedApril 13, 2021, 1:15 p.m....resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...